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Tobacco acid pyrophosphatase : ウィキペディア英語版 | Tobacco acid pyrophosphatase
Tobacco Acid Pyrophosphatase (TAP) is an enzyme that catalyses the hydrolysis of a phosphoric ester bond in a broad spectrum of molecules, including the 5'-end of mRNA. During mRNA maturation the 5' triphosphate of the new mRNA molecule is rapidly removed. The diphosphate 5' end then attacks the α-phosphorus atom of a methylated GTP to form a very unusual 5'-5' triphosphate linkage, called ''cap''.〔(Online version )〕 In molecular biology, TAP is used to hydrolyse a phosphodiester bond of this particular structure and release a mRNA molecule with only one phosphate group in the 5'-end, for instance in protocols for RACE (Rapid Amplification of cDNA Ends). == References ==
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Tobacco acid pyrophosphatase」の詳細全文を読む
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